Pregnant SD rats were randomly dived into four groups: sham group (n = 8), PE group (n = 8), PE + ferrostatin-1 group ( n= 8), and PE + miR-30b-5p inhibition group (n = 8). On day 14 of pregnancy, PE rat model was established through reduced uterine perfusion pressure (RUPP) surgery, wherein constrictive silver clips were placed on the aorta (0.203-mm clips) superior to the iliac bifurcation and on the ovarian vessels (0.100-mm clips), according to a previous description. Sham rats were operated on in a fashion similar to that of RUPP rats, but without clipping. Mini-pumps were also intraperitoneally inserted into rats on day 14 of pregnancy. The mini-pump in each rat delivered the ferrostatin-1 at a dose of 10 umol/kg/day for 5 days. An miR-30b-5p antagonist (GenePharma) was injected from the tail veins on day 13 of gestation, at a rate of 100 uL/day for 6 days. The blood pressure was measured on days 14, 16, and 19 of gestation using catheters inserted into the carotid artery and jugular vein.

Pregnant SD rats were randomly dived into four groups: sham group (n = 8), PE group (n = 8), PE + ferrostatin-1 group ( n= 8), and PE + miR-30b-5p inhibition group (n = 8). On day 14 of pregnancy, PE rat model was established through reduced uterine perfusion pressure (RUPP) surgery, wherein constrictive silver clips were placed on the aorta (0.203-mm clips) superior to the iliac bifurcation and on the ovarian vessels (0.100-mm clips), according to a previous description. Sham rats were operated on in a fashion similar to that of RUPP rats, but without clipping. Mini-pumps were also intraperitoneally inserted into rats on day 14 of pregnancy. The mini-pump in each rat delivered the ferrostatin-1 at a dose of 10 umol/kg/day for 5 days. An miR-30b-5p antagonist (GenePharma) was injected from the tail veins on day 13 of gestation, at a rate of 100 uL/day for 6 days. The blood pressure was measured on days 14, 16, and 19 of gestation using catheters inserted into the carotid artery and jugular vein.

Pregnant SD rats were randomly dived into four groups: sham group (n = 8), PE group (n = 8), PE + ferrostatin-1 group ( n= 8), and PE + miR-30b-5p inhibition group (n = 8). On day 14 of pregnancy, PE rat model was established through reduced uterine perfusion pressure (RUPP) surgery, wherein constrictive silver clips were placed on the aorta (0.203-mm clips) superior to the iliac bifurcation and on the ovarian vessels (0.100-mm clips), according to a previous description. Sham rats were operated on in a fashion similar to that of RUPP rats, but without clipping. Mini-pumps were also intraperitoneally inserted into rats on day 14 of pregnancy. The mini-pump in each rat delivered the ferrostatin-1 at a dose of 10 umol/kg/day for 5 days. An miR-30b-5p antagonist (GenePharma) was injected from the tail veins on day 13 of gestation, at a rate of 100 uL/day for 6 days. The blood pressure was measured on days 14, 16, and 19 of gestation using catheters inserted into the carotid artery and jugular vein.

Pregnant rats were randomly divided into 3 groups on gestational day 13, with 8 in each group: Sham group, PE group, and PE + ferrostatin-1 (FI) group. As previously mentioned, on gestational day 14, rats in the PE and PE + FI groups were subjected to reduced uterine perfusion pressure (RUPP) surgery to establish the PE model. Briefly, pregnant rats were anesthetized, sterilized with iodophor, and then opened. A contractile silver clip (0.203 mm) was placed on the aorta above the iliac bifurcation, as well as the bilateral uterine arcades at the end of the ovary were reduced with restrictive clips (0.100 mm) to the ovarian collaterals of the uterus. The rats in the Sham group were operated in a manner similar to the RUPP rats but were not clipped. On a gestational day 14, the mini-pumps were also inserted into the rat's peritoneum. The mini-pump in each rat of the PE + FI group was used to deliver FI at a dose of 10 mol/kg/d for 5 d. Systolic and diastolic blood pressure (SBP and DBP) were measured on gestational days 14, 16, and 19 using catheters inserted into the carotid artery and jugular vein. On a gestational day 19, urine, blood, and placenta of rats were collected for subsequent experiments. Urine protein content was detected using a commercial kit (C035-2, Nanjing Jiancheng, China). Plasma sFlt-1 content was detected by a commercial kit (ml059017, Mlbio, China). The remaining samples were stored at -80 .