Ferroptosis Regulator Information
General Information of the Ferroptosis Regulator (ID: REG30052)
Full List of the Ferroptosis Target of This Regulator and Corresponding Disease/Drug Response(s)
PMAN
can regulate the following target(s), and cause disease/drug response(s). You can browse detail information of target(s) or disease/drug response(s).
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Cystine/glutamate transporter (SLC7A11) [Driver; Suppressor]
| In total 1 item(s) under this target | |||||
| Experiment 1 Reporting the Ferroptosis Target of This Regulator | [1] | ||||
| Target for Ferroptosis | Suppressor | ||||
| Responsed Disease | Gastric cancer | ICD-11: 2B72 | |||
| Pathway Response | Ferroptosis | hsa04216 | |||
| Cell Process | Cell ferroptosis | ||||
| Cell proliferation | |||||
In Vitro Model |
AGS cells | Gastric adenocarcinoma | Homo sapiens | CVCL_0139 | |
| MGC-803 cells | Gastric mucinous adenocarcinoma | Homo sapiens | CVCL_5334 | ||
| In Vivo Model |
For animal models of gastric subserosal injection, we collected MGC-803 cell lines (5 x 10 cells) that were infected by lentivirus with or without PMAN-OE, and suspended in 40 ul serum-free medium (50% Matrigel). After that, nude mice (six mice per group) were anesthetized by intraperitoneal injection of 100 ul of pentobarbital (1%). After disinfection, the abdominal cavity was opened to expose the greater curvature of the stomach. The tumor suspension (40 ul) was implanted under the serosa of the greater curvature of the stomach of the nude mice through an insulin needle.
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| Response regulation | HIF-1 could act as a protective factor against ferroptosis in gastric cancer (GC) cells. HIF-1 activates PMAN at the transcriptional level, which greatly improves the output of ELAVL1 in the cytoplasm. ELAVL1 directly combines with the AREs of SLC7A11 mRNA 3-UTR and improves the stability ofSLC7A11mRNA, thereby increasing the expression of SLC7A11 and reducing the accumulation of ROS and iron in ferroptosis, ultimately promoting the proliferation and development of tumor cells. | ||||
Gastric cancer [ICD-11: 2B72]
| In total 1 item(s) under this disease | |||||
| Experiment 1 Reporting the Ferroptosis-centered Disease Response | [1] | ||||
| Target Regulator | PMAN (IncRNA) | lncRNA | |||
| Pathway Response | Ferroptosis | hsa04216 | |||
| Cell Process | Cell ferroptosis | ||||
| Cell proliferation | |||||
In Vitro Model |
AGS cells | Gastric adenocarcinoma | Homo sapiens | CVCL_0139 | |
| MGC-803 cells | Gastric mucinous adenocarcinoma | Homo sapiens | CVCL_5334 | ||
| In Vivo Model |
For animal models of gastric subserosal injection, we collected MGC-803 cell lines (5 x 10 cells) that were infected by lentivirus with or without PMAN-OE, and suspended in 40 ul serum-free medium (50% Matrigel). After that, nude mice (six mice per group) were anesthetized by intraperitoneal injection of 100 ul of pentobarbital (1%). After disinfection, the abdominal cavity was opened to expose the greater curvature of the stomach. The tumor suspension (40 ul) was implanted under the serosa of the greater curvature of the stomach of the nude mice through an insulin needle.
Click to Show/Hide
|
||||
| Response regulation | HIF-1 could act as a protective factor against ferroptosis in gastric cancer (GC) cells. HIF-1 activates PMAN at the transcriptional level, which greatly improves the output of ELAVL1 in the cytoplasm. ELAVL1 directly combines with the AREs of SLC7A11 mRNA 3-UTR and improves the stability ofSLC7A11mRNA, thereby increasing the expression of SLC7A11 and reducing the accumulation of ROS and iron in ferroptosis, ultimately promoting the proliferation and development of tumor cells. | ||||
References