Male c57BL/6 mice (8 weeks old) were purchased from Beijing Wei Tong Li Hua Experimental Animal Technology Co. Ltd. (Beijing, China). Mice were divided into control (n = 8), LPS (n = 9), and VitB6+LPS (n = 9) groups. Mice were pretreated with PBS or VitB6 for 6 h and then treated with LPS (4 mg/kg) for 24 h. Cardiac ultrasound was performed before sacrifice. Inhaled isoflurane was given to mice for volatile anesthesia and the chest hair was removed with a depilatory cream. Then, mice were fixed on the warmed imaging platform and wore with the coupling agent. The Vevo2100 imaging system, equipped with a 40-MHz high-frequency transducer (VisualSonics Inc., Toronto, Canada), was applied to perform non-invasive examinations. The M-mode echocardiogram at the parasternal long axis was used to obtain the ejection fraction (EF) of left ventricular and fractional shortening (FS).

Six-week-old C57BL/6 male mice were randomly divided into two groups; the first group was fed a 45% high fat diet for 20 weeks while the control group were fed a normal chow diet. ATM-exosomes isolated from obese and lean mice were transferred into normal mice via tailvein injection(30 ug per week), as previously described. To investigate glutathione synthesis, 20 mM l-buthionine-(S,R)-sulfoximine (BSO) was added to the drinking water and administered to mice for 2 weeks. To inhibit ferroptosis, mice were given an intraperitoneal injection with Fer-1 (1 mg/kg).