Ferroptosis Regulator Information
General Information of the Ferroptosis Regulator (ID: REG20065)
Full List of the Ferroptosis Target of This Regulator and Corresponding Disease/Drug Response(s)
mmu-miR-7212-5p
can regulate the following target(s), and cause disease/drug response(s). You can browse detail information of target(s) or disease/drug response(s).
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Heme oxygenase 1 (HMOX1) [Driver; Suppressor]
| In total 1 item(s) under this target | |||||
| Experiment 1 Reporting the Ferroptosis Target of This Regulator | [1] | ||||
| Target for Ferroptosis | Suppressor | ||||
| Responsed Disease | Sepsis | ICD-11: 1G40 | |||
| Pathway Response | Ferroptosis | hsa04216 | |||
| Cell Process | Cell ferroptosis | ||||
In Vitro Model |
TCMK-1 cells | Normal | Mus musculus | CVCL_2772 | |
| In Vivo Model |
C57BL/6 mice (male, 6-8 weeks old) were purchased from Guangdong Yaokang Biotechnology Co., Ltd. (China). According to a common sepsis protocol, we used the cecal ligation and puncture (CLP) method to construct a model of sepsis. For RNA sequencing, 5 mice were randomly divided into 2 groups: the CLP group (n = 3) and the sham group (n = 2). We anesthetized mice in the CLP group with 24% isoflurane. Under aseptic conditions, a 2-cm midline laparotomy was created below the diaphragm to expose the cecum. Two-thirds of the cecum was ligated with a 5-0 silk suture and punctured twice using a 22-gauge needle. The cecum was gently squeezed to extrude a small amount of feces through the perforation site. Animals were resuscitated with 1 mL of subcutaneous saline after CLP. The procedures of the sham group (controls) were the same as the CLP group, except for the ligation and perforation. The mice were sacrificed via neck fracture 6 hours after CLP, and the kidneys were taken for subsequent RNA sequencing.
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| Response regulation | The mmu-miR-7212-5p-Hmox1 axis in ferroptosis and m6A RNA methylation regulators may have potential clinical significance for the future treatment of Sepsis-associated acute kidney injury (SA-AKI). | ||||
Sepsis [ICD-11: 1G40]
| In total 1 item(s) under this disease | |||||
| Experiment 1 Reporting the Ferroptosis-centered Disease Response | [1] | ||||
| Target Regulator | mmu-miR-7212-5p (miRNA) | miRNA | |||
| Pathway Response | Ferroptosis | hsa04216 | |||
| Cell Process | Cell ferroptosis | ||||
In Vitro Model |
TCMK-1 cells | Normal | Mus musculus | CVCL_2772 | |
| In Vivo Model |
C57BL/6 mice (male, 6-8 weeks old) were purchased from Guangdong Yaokang Biotechnology Co., Ltd. (China). According to a common sepsis protocol, we used the cecal ligation and puncture (CLP) method to construct a model of sepsis. For RNA sequencing, 5 mice were randomly divided into 2 groups: the CLP group (n = 3) and the sham group (n = 2). We anesthetized mice in the CLP group with 24% isoflurane. Under aseptic conditions, a 2-cm midline laparotomy was created below the diaphragm to expose the cecum. Two-thirds of the cecum was ligated with a 5-0 silk suture and punctured twice using a 22-gauge needle. The cecum was gently squeezed to extrude a small amount of feces through the perforation site. Animals were resuscitated with 1 mL of subcutaneous saline after CLP. The procedures of the sham group (controls) were the same as the CLP group, except for the ligation and perforation. The mice were sacrificed via neck fracture 6 hours after CLP, and the kidneys were taken for subsequent RNA sequencing.
Click to Show/Hide
|
||||
| Response regulation | The mmu-miR-7212-5p-Hmox1 axis in ferroptosis and m6A RNA methylation regulators may have potential clinical significance for the future treatment of Sepsis-associated acute kidney injury (SA-AKI). | ||||