Ferroptosis-centered Disease Response Information

General Information of the Disease (ID: DIS00071)
Name
Multiple sclerosis
ICD
ICD-11: 8A40
Full List of Target(s) of This Ferroptosis-centered Disease
Cystine/glutamate transporter (SLC7A11)
 Target Info 
In total 2 item(s) under this target
Experiment 1 Reporting the Ferroptosis-centered Disease Response by This Target [1]
Target for Ferroptosis Suppressor
Responsed Disease Multiple sclerosis [ICD-11: 8A40]
Responsed Regulator hsa-miR-367-3p (miRNA) Suppressor
 Regulator Info 
Pathway Response Fatty acid metabolism hsa01212
Ferroptosis hsa04216
Cell Process Cell ferroptosis
In Vitro Model hBMSCs (Bone marrow stromal cells)
BV-2 cells Normal Mus musculus CVCL_0182
In Vivo Model
Female C57BL6 mice were randomized into four groups (n = 8/group): the control group (non-EAE mice), the EAE group (EAE mice injected intrathecally with 5 uL PBS when symptoms appeared), the EAE + BMSCs-Exo + mimic negative control (NC) group (EAE mice injected intrathecally with Exos from bone MSCs (BMSCs) transfected with mimic NC [5 uL, 2 ug/L in PBS] when symptoms appeared), and the EAE + BMSCs-Exo + miR-367-3p mimic group (EAE mice injected intrathecally with Exos from BMSCs transfected with miR-367-3p mimic [5 uL, 2 ug/L in PBS] when symptoms appeared).

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Response regulation MiR-367-3p can be delivered by BMSC-Exos into microglia, where miR-367-3p inhibits EZH2 expression and activates the expression of SLC7A11, suppressing microglial ferroptosis and relieving the symptoms of EAE. Experimental autoimmune encephalomyelitis (EAE) is a typical animal model of multiple sclerosis.
Experiment 2 Reporting the Ferroptosis-centered Disease Response by This Target [1]
Target for Ferroptosis Suppressor
Responsed Disease Multiple sclerosis [ICD-11: 8A40]
Responsed Regulator Histone-lysine N-methyltransferase EZH2 (EZH2) Driver
 Regulator Info 
Pathway Response Fatty acid metabolism hsa01212
Ferroptosis hsa04216
Cell Process Cell ferroptosis
In Vitro Model hBMSCs (Bone marrow stromal cells)
BV-2 cells Normal Mus musculus CVCL_0182
In Vivo Model
Female C57BL6 mice were randomized into four groups (n = 8/group): the control group (non-EAE mice), the EAE group (EAE mice injected intrathecally with 5 uL PBS when symptoms appeared), the EAE + BMSCs-Exo + mimic negative control (NC) group (EAE mice injected intrathecally with Exos from bone MSCs (BMSCs) transfected with mimic NC [5 uL, 2 ug/L in PBS] when symptoms appeared), and the EAE + BMSCs-Exo + miR-367-3p mimic group (EAE mice injected intrathecally with Exos from BMSCs transfected with miR-367-3p mimic [5 uL, 2 ug/L in PBS] when symptoms appeared).

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Response regulation MiR-367-3p can be delivered by BMSC-Exos into microglia, where miR-367-3p inhibits EZH2 expression and activates the expression of SLC7A11, suppressing microglial ferroptosis and relieving the symptoms of EAE. Experimental autoimmune encephalomyelitis (EAE) is a typical animal model of multiple sclerosis.
References
Ref 1 Mesenchymal stem cell-derived exosomal microRNA-367-3p alleviates experimental autoimmune encephalomyelitis via inhibition of microglial ferroptosis by targeting EZH2. Biomed Pharmacother. 2023 Jun;162:114593. doi: 10.1016/j.biopha.2023.114593. Epub 2023 Mar 29.