Ferroptosis-centered Disease Response Information
General Information of the Disease (ID: DIS00037)
| Name |
Merkel cell carcinoma
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| ICD |
ICD-11: 2C34
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Full List of Target(s) of This Ferroptosis-centered Disease
Unspecific Target
| In total 1 item(s) under this target | |||||
| Experiment 1 Reporting the Ferroptosis-centered Disease Response by This Target | [1] | ||||
| Responsed Disease | Merkel cell carcinoma [ICD-11: 2C34] | ||||
| Responsed Drug | Artesunate | Investigative | |||
| Pathway Response | Fatty acid metabolism | hsa01212 | |||
| Ferroptosis | hsa04216 | ||||
| Cell Process | Cell ferroptosis | ||||
| Cell proliferation | |||||
| In Vitro Model | MKL-1 cells | Merkel cell carcinoma | Homo sapiens | CVCL_2600 | |
| MKL-2 cells | Merkel cell carcinoma | Homo sapiens | CVCL_D027 | ||
| MS-1 cells | Lung small cell carcinoma | Homo sapiens | CVCL_1429 | ||
| WaGa cells | Merkel cell carcinoma | Homo sapiens | CVCL_E998 | ||
| PeTa cells | Merkel cell carcinoma | Homo sapiens | CVCL_LC73 | ||
| In Vivo Model |
Five-week-old female NOD.CB17/Prkdcscid mice (Charles River) were used for the xenotransplantation experiments. They were housed under specific pathogen-free conditions. Each mouse was injected subcutaneously with a suspension of 5 x 106 MKL-1 or WaGa tumor cells mixed with an equal volume of Matrigel (Corning) in a total volume of 100 uL. The tumor size was measured daily using a vernier calipers.
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| Response regulation | Artesunate predominantly induces ferroptosis in MCPyV-positive merkel cell carcinoma (MCC) cells since known ferroptosis-inhibitors like DFO, BAF-A1, Fer-1 and -mercaptoethanol reduced artesunate-induced death. Rosiglitazone (Rosi), an inhibitor of the Acyl-CoA synthetase long-chain family member 4 (ACSL4). Indeed, Rosi exerted a protective effect on all three tested artesunate-treated MCC cell lines. | ||||