Ferroptosis-centered Disease Response Information
General Information of the Disease (ID: DIS00036)
| Name |
Squamous cell carcinoma of skin
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| ICD |
ICD-11: 2C31
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Full List of Target(s) of This Ferroptosis-centered Disease
Long-chain-fatty-acid--CoA ligase 4 (ACSL4)
| In total 1 item(s) under this target | |||||
| Experiment 1 Reporting the Ferroptosis-centered Disease Response by This Target | [1] | ||||
| Target for Ferroptosis | Driver | ||||
| Responsed Disease | Cutaneous squamous cell carcinoma [ICD-11: 2C31] | ||||
| Responsed Drug | Itraconazole | Investigative | |||
| Responsed Regulator | Hydroxymethylglutaryl-CoA synthase, cytoplasmic (HMGCS1) | Driver | |||
| Pathway Response | Fatty acid metabolism | hsa01212 | |||
| Ferroptosis | hsa04216 | ||||
| Apoptosis | hsa04210 | ||||
| Cell Process | Cell ferroptosis | ||||
| Cell apoptosis | |||||
| Cell proliferation | |||||
| In Vitro Model | A-431 cells | Skin squamous cell carcinoma | Homo sapiens | CVCL_0037 | |
| COLO 16 cells | Skin squamous cell carcinoma | Homo sapiens | CVCL_D607 | ||
| In Vivo Model |
Female BALB/c nude mice (6weeks old and 18-22 g weight) were purchased from the Model Animal Research Center of Nanjing University. A431 cells (5 x 106) in cold DMEM (50 ul) were mixed with Matrigel (50 ul) and injected into mice subcutaneously. After 6 days, the tumor volume was measured and the mice were assigned to three groups. Mice were treated with either normal saline or itraconazole (40 mg/kg oral twice daily; 80 mg/kg oral twice daily).
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| Response regulation | Itraconazole inhibited the cell proliferation, induced apoptosis and blocked cell cycle of Cutaneous squamous cell carcinoma cells. And 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) and acyl-CoA synthetase long-chain family member 4 (ACSL4) were significantly upregulated in A431 cells treated with itraconazole. Itraconazole may induce ferroptosis via HMGCS1/ACSL4 axis in A431 cells. | ||||